Practical identification of eight medically importantTrichosporonspecies by reverse line blot hybridization (RLB) assay and rolling circle amplification (RCA)
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چکیده
منابع مشابه
Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Multiplex PCR/Reverse Line Blot Hybridization assay allows the detection of up to 43 molecular targets in 43 samples using one multiplex PCR reaction followed by probe hybridization on a nylon membrane, which is re-usable. Probes are 5' amine modified to allow fixation to the membrane. Primers are 5' biotin modified which allows detection of hybridized PCR products using streptavidin-peroxidase...
متن کاملReverse line blot hybridization assay for identification of medically important fungi from culture and clinical specimens.
We evaluated a combined panfungal PCR-reverse line blot (RLB) hybridization assay based on internal transcribed spacer 1 (ITS1) and ITS2 region polymorphisms to identify 159 Candida, Cryptococcus neoformans, and Aspergillus isolates (22 species). Its utility to identify fungal pathogens directly from 27 clinical specimens was also determined. ITS sequence analysis was performed to resolve discr...
متن کاملRolling Circle Amplification (RCA): an approach for quick detection and identification of fungal species
Conventional methods for fungal identification in the clinical laboratory rely on morphological andphysiological tests. These tests often need several days or weeks to complete and are frequentlyunspecific. Molecular identification mostly implies sequencing, which is relatively expensive andtime-consuming, as well as impractical for large numbers of isolates. The Rolling CircleAmplification app...
متن کاملDetection and identification of eight Trichinella genotypes by reverse line blot hybridization.
A reverse line blot (RLB) assay was developed to identify different Trichinella genotypes. The RLB assay accomplishes detection and specific identification of the different Trichinella genotypes and relies on hybridization of the amplified 5S ribosomal DNA intergenic spacer regions to specific, membrane-bound oligonucleotide probes. After one single amplification, we were able to detect and gen...
متن کاملrolling circle amplification (rca): an approach for quick detection and identification of fungal species
conventional methods for fungal identification in the clinical laboratory rely on morphological andphysiological tests. these tests often need several days or weeks to complete and are frequentlyunspecific. molecular identification mostly implies sequencing, which is relatively expensive andtime-consuming, as well as impractical for large numbers of isolates. the rolling circleamplification app...
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ژورنال
عنوان ژورنال: Medical Mycology
سال: 2013
ISSN: 1369-3786,1460-2709
DOI: 10.3109/13693786.2012.723223